Fig. 1

NT-3 upregulates the expression of c-Jun in Schwann cells. a Western blotting was used to detect the expression of c-Jun in the uninjured and injured distal stumps after 4 days and at 1, 3, 5, 8, and 10 weeks of denervation. b Quantification of the relative expression of c-Jun in a. Data were normalized to 3 weeks post-injury. One-way ANOVA with Dunnett’s multiple comparisons test, ****P < 0.0001, n = 3. c Western blot of c-Jun in cultured Schwann cells at passages 2, 4, 6, and 8. d Quantification of the relative expression of c-Jun in c. Data were normalized to passage 2. One-way ANOVA with Dunnett’s multiple comparisons test, **P < 0.005, ****P < 0.0001, n = 3. e The expression of c-Jun at different time points after administration of NT-3 analyzed using western blotting. f Relative quantitative analysis of c-Jun expression shows that NT-3 could upregulate c-Jun expression. Data normalized to 0 (control group). One-way ANOVA with Dunnett’s multiple comparisons test, *P < 0.05, **P < 0.005, n = 3. g Immunofluorescence analysis of c-Jun expression in Schwann cells; scale bar, 50 μm. h The expression of c-Jun in Schwann cells analyzed using fluorescence intensity. The expression of c-Jun in the NT-3 group is significantly upregulated compared to that in the control group. Unpaired Student’s t-test, **P < 0.005, n = 4. i Percentage of c-Jun positive Schwann cells. Unpaired Student’s t-test, ***P < 0.001, n = 4. All numerical data are presented as mean ± SEM