Fig. 2

Characterization of the ASC bioproducts. A FACS analysis using the MACSPlex exosomal kit. EV-UC, EV-SEC, Protein-rich fraction, CM and CM-WO were tested for 39 surface markers divided into groups based on their origin and function (embryonic cells, pro-coagulation activity, neurite growth, cell adhesion, hematopoietic cells, immune system regulation, immune cells, mesenchymal cells, tetraspanins and two controls). Data represented as a heatmap with the mean fluorescence intensity of N = 3 ASC donors per marker. B Representative super-resolution microscopy pictures of EV-UC, EV-SEC, CM. The samples are stained with CD81 red, CD44 green and CD9 blue. The scale bar is 100 nm. C Protein content analysis showed higher concentration of protein in CM and Protein-Rich Fraction preparations and lower in EV-UC. No protein content was detected in the EV-SEC or the CM-WO groups. D Western blot analysis of Calnexin of EV-UC, EV-SEC, Protein-rich fraction, CM-WO, CM. Cell lysates of Hela cells were used as a positive control