Fig. 1
a APs of neurons: The amplitude and frequency of neuronal AP in the Mg2 + -free group were higher than those in the control group (n = 6 in each group, # p < 0.01). b mIPSCs of neurons: Compared with that in the control group, the frequency of mIPSCs in the Mg2 + -free group did not change significantly, while the amplitude of mIPSCs in the Mg2 + -free group was decreased (n = 6 in each group, * p < 0.05). c Total protein expression (in vitro): Compared with that in the control group, NPTN expression in the Mg2 + -free group was decreased (n = 6 in each group, #p < 0.01), while GABRA1 and VGAT expression did not significantly change. d Surface protein expression (in vitro): Surface GABRA1 in the Mg2 + -free group was decreased compared with that in the control group (n = 5 in each group, #p < 0.01). e Rat EEG recordings: No significant seizures and epileptic discharges were recorded in the control group. Contrarily, EEG recordings showed recurrent epileptic discharges during the interictal period and generalized multi-spike waves and multi-spike waves complexes during the ictal period in all SE groups. time scale: 5 s. f Total protein expression (in vivo): Compared with that in the control group, NPTN expression in the SE group was decreased (n = 6 in each group, #p < 0.01), while the changes in GABRA1 and VGAT expression were not significant. g Surface protein expression (in vivo): Surface GABRA1 in the SE group was decreased compared with that in the control group (n = 6 in each group, *p < 0.05). h Internalization assay (× 600): The intracellular accumulation ratio of neurons in the Mg2 + -free group was increased compared with that of those in the control group (intracellular accumulation ratioCrtl = 0.23 ± 0.02, intracellular accumulation ratioMg2+-free = 0.70 ± 0.04, n = 9 in each group, # p < 0.01). All experiments were repeated for 3 times. △Independent t-tests were used for the comparison of means