Fig. 2

SUMOylation promotes the nuclear translocation of vimentin in PDCs. A Western blot analysis of SUMOylated vimentin-related total cell protein levels (PIAS1, SUMO1, and SUMO2/3). B Western blot analysis of SUMOylated vimentin-related cytoplasmic protein levels (PIAS1, SUMO1, and SUMO2/3). C Western blot analysis of SUMOylated vimentin-related nuclear protein levels (PIAS1, SUMO1, and SUMO2/3). D Results of vimentin coimmunoprecipitation in Hct116 control cells and Hct116 PDCs (anti-vimentin antibody was used for immunoprecipitation). E Results of vimentin co-immunoprecipitation in LoVo control cells and LoVo PDCs (an anti-vimentin antibody was used for immunoprecipitation). F Total expression of SUMO1 and vimentin in Hct116 PDCs after transfection with three siRNA-SUMO1 sequences (307, 358, and 727). G Total expression of SUMO1 and vimentin in LoVo PDCs after transfection with three siRNA-SUMO1 sequences (307, 358, and 727). SUMO small ubiquitin-like modification, PDCs polyploid giant cancer cells with daughter cells