Fig. 6
CaMKIIα neurons in the LPBN modulate pain responses and aversion. A Duration of licking in CaMKIIαmCherry and CaMKIIαhM4Di mice following formalin intraplantar injection. B–D The effect of chemogenetic inhibition of CaMKIIα neurons in the LPBN on CPA induced by intraplantar formalin injection. B Experimental design for CPA. CNO (i.p., 2.5 mg/kg) was given 30 min prior to 2% intraplantar formalin on Days 2 and 3, which was paired with one side of a two-chambered box differentiated by visual cues. C, D Examples of tracking maps and quantification of time spent in the stimulated side in the CPA before (Pre) (C) and after (Post) (D) injection of CNO into CaMKIIαmCherry mice and CaMKIIαhM4Di mice. E, G Chemogenetic activation of CaMKIIα neurons in the LPBN induced CPA. E Experimental design for CPA. CNO (i.p., 2.5 mg/kg) was given 30 min prior to training on Days 2 and 3, which was paired with one side of a two-chambered box differentiated by visual cues. F, G Examples of tracking maps and quantification of time spent in the stimulated side in the CPA before (Pre) (F) and after (Post) (G) injection of CNO into CaMKIIαmCherry mice and CaMKIIαhM3Dq mice. Data are mean ± SEM. *p < .05. Two-way ANOVA followed by the Sidak’s multiple comparisons test in A. Two-tailed unpaired t test in C, D, F and G