Fig. 1

Formalin-induced acute inflammatory pain impairs 3CSRTT performance. A Schematic of the formalin test. Paw injection of 2% formalin was administered at 0 min; the time spent licking the paw was measured for 60 min. B The time spent licking the paw following saline (n = 5 mice) or formalin (n = 7 mice) injection displayed in 5-min time bins. C Experimental design. 3CSRTT was performed immediately after formalin injection for 60 min. D–G Effects of saline or formalin injection on correct (D), omission (E), incorrect (F), and premature (G) responses in the 3CSRTT over the 60-min testing period. Data points are displayed in 5-min time bins. H–K Effects of saline or formalin injection on correct (H), omission (I), incorrect (J), and premature (K) responses in the 3CSRTT in phases 1 and 2. L, M Effects of saline or formalin injection on correct response latency (L) and reward latency (M) in the 3CSRTT (n = 7 mice per group). Data are mean ± SEM. *p < .05, **p < .01, ***p < .001. Two-way ANOVA followed by the Sidak’s multiple comparisons test in B, D–G. Two-tailed unpaired t test in H–M