Fig. 5

The LINC02913/IGF1R axis affected the phenotype of ADSCs under hypoxia via regulating the PI3K/AKT pathway. A IGF1R mRNA expression in ADSCs cultured under different oxygen concentrations was detected using qRT-PCR; B IGF1R protein level in ADSCs cultured under different oxygen concentrations was detected using western blot; C the effect of the LINC02913/IGF1R axis on IGF1R expression under hypoxia was detected through a series of rescue experiments using western blot; D the effect of the LINC02913/IGF1R axis on the PI3K/AKT pathway-related protein was detected using western blot; E the effect of the LINC02913/IGF1R axis on cell viability under hypoxia was detected using CCK-8 assay; F cell adipogenic ability under hypoxia was detected using oil red O staining; G after the endothelial differentiation of ADSCs, the effect of the LINC02913/IGF1R axis on cell tube formation under hypoxia was detected; H the effect of the LINC02913/IGF1R axis on the expression of CD31 (endothelial cell marker) was detected using IF staining; I the effect of the LINC02913/IGF1R axis on the level of endothelial cell markers CD133, CD31, VEGFA, and eNOS were detected using western blot. *p < 0.05, **p < 0.01, compared with the vector group; ##p < 0.01, compared with the LINC02913 OE group