Fig. 9

Role of RNF149 in Modulating CD8⁺ T Cell Evolution.A–D. Trajectories derived from Monocle 2 depict dynamic shifts in CD8⁺ T cell subclusters (A), subpopulations (B), temporal progression (C), and RNF149 expression (D). E Monocle 3 trajectory highlights CD8⁺ T cell subclusters. F Density plot delineating the temporal differentiation of three CD8⁺ T cell subpopulations. G Hierarchical clustering heatmap reveals marker genes linked with developmental phases and cell subpopulations. H Differential hierarchical clustering heatmap indicates marker genes pertinent to developmental stages and cellular subsets within differentiation lineage "branch-3."