Fig. 6

Knockdown of FABP5 reverses the proliferation, migration, and invasion of cells with ALKBH5 over-expression. A qRT-PCR and B Western blotting were performed to examine FABP5 expression in different transfected cell groups. C–I Rescue experiments by CCK-8 (C), colony formation (D, E), EdU assays (F, G), and Transwell assays (H, I) were conducted to evaluate the effect of FABP5 interference on the growth and motility of QGP-1 cells with over-expressed ALKBH5. J–N Rescue experiments were performed in QGP-1 cells with over-expressed ALKBH5 to evaluate the effect of FABP5 interference on the amounts of LDs (lipid droplets) (J, K), FFA (free fatty acids) (L), total cholesterol level (M), and triglyceride (N). O–R Representative subcutaneous xenograft tumor image from the indicated groups (O). Tumors were removed 4 weeks after Subcutaneous implantation, followed by volume calculation and weight measurement (P, Q). Representative IHC staining images of ALKBH5, FABP5, and Ki67 (R). *p < 0.01, **p < 0.01, ***p < 0.001, ****p < 0.0001