Fig. 2

ALKBH5 knockdown inhibits proliferation, migration, and invasion of QGP-1 cells in vitro and in vivo. A, B The mRNA and protein expression of ALKBH5 in QGP-1 cells were assessed by qRT-PCR and western blotting (Scr represents scramble which means control group with disrupted rna sequence; Oc represents overexpression control). C, D CCK-8 proliferation assays were carried out in QGP-1 cells with ALKBH5 knockdown and overexpression. E, F Colony formation assays were conducted in QGP-1 cells with ALKBH5 knockdown and overexpression. Column diagrams showed the relative colony numbers of each group. G–J EdU assays were carried out to evaluate the proliferation of cells with ALKBH5 knockdown and overexpression and a positive rate of EdU was calculated, magnification: ×200. K–N Representative images of transwell assays to evaluate the migration and invasion capacity of QGP-1 cells with ALKBH5 knockdown and overexpression, magnification: ×100. Quantification data showed the relative transwell numbers of cells which passed through the chamber membrane. O–Q Representative images of tumors and comparison of the tumor volume and weight between ALKBH5-deficient groups and scramble groups in QGP-1 cells. *p < 0.01, **p < 0.01, ***p < 0.001