Fig. 7

FMD inhibits the secretion of CCL8 by TAMs. A Serum CCL8 level in mice is detected by ELISA (*P < 0.05, vs. respective control group, ^#P < 0.05, vs. respective TAM group). B CCL8 secretion from TAMs treated for 24 h with GD or EVE (100 nM) or 2-DG (10 mM), or 2ME2 (10 µM) is determined by ELISA (*P < 0.05, vs. respective TAM group). C CCL8 mRNA levels in TAMs are determined by RT-PCR (*P < 0.05, vs. respective TAM group). D Migration of MDA-MB-231 cells, after co-incubation with TAMs and low (L) or high doses (H) of CCL8 antibody, is determined in a transwell assay (magnification, ×40)