Fig. 4

FMD inhibits mTOR phosphorylation in TAMs. A mTOR and HIF1-α expressions in tumor tissues are detected using western blotting. B, C mTOR and HIF1-α in TAMs treated with GD or EVE (100 nM) for 24 h, are detected using western blotting. D Immunofluorescence images of HIF-1α expression in hypoxic TAMs (magnification, ×40). Data are presented as the means ± S.E.M. from three separate experiments. *P < 0.05, **P < 0.01, vs. respective control group; ^#P < 0.05 vs. hypoxia group; n.s no significant difference