Fig. 3

FMD inhibits the survival and polarization of hypoxic TAMs. A The schematic diagram of detection of the survival, polarization, tumor-promoting function of hypoxic TAMs after treatment with glucose deficiency. B The cell viabilities of glucose-deprived TAMs, and those treated with WZB117 (10 µM), and 2-DG (10 mM) for 72 h are determined by CCK-8 assay. C Representative images of TAMs morphology in hypoxia (magnification, ×40). D The mRNA expression of Arg1, CD163, CD206, and IL-10 in TAMs under hypoxia are measured by RT-PCR. E Expressions of CD 86 and CD 163 of TAMs cells are analyzed by immunofluorescence (magnification, ×40). F Migration of MDA-MB-231 cells after co-incubation for 24 h with CM of TAMs is determined using scratch-wound assays. Control group: MDA-MB-231 co-incubation with CM of TAMs; Hypoxia group: MDA-MB-231 co-incubation with CM of hypoxic TAMs; Hypoxia + 50% GD: MDA-MB-231 co-incubation with CM of 50% GD-treated hypoxic TAMs (magnification, ×10). G Migration of MDA-MB-231 cells after co-incubation with TAMs for 24 h is determined in Transwell assays. Blank group: MDA-MB-231 incubation without TAMs; Control group: MDA-MB-231 co-incubation with TAMs; Hypoxia group: MDA-MB-231 co-incubation with hypoxic TAMs; Hypoxia + 50% GD: MDA-MB-231 co-incubation with 50% GD-treated hypoxic TAMs (magnification, ×10). Data are presented as the means ± S.E.M. from three separate experiments. *P < 0.05, **P < 0.01, vs. respective TAM group