Fig. 7
From: Insulin-induced gene 2 protects against hepatic ischemia–reperfusion injury via metabolic remodeling
The PPP mediates the effect of Insig2 on hepatic IR injury. Mice injected with AAV8-NC and AAV8-Insig2 were treated with DMSO or 6-AN, followed by hepatic IR injury. A, B Levels of serum ALT and AST (n = 3/group). C Representative HE staining and quantification of necrotic areas (n = 3/group). D Expression of apoptosis signaling pathway proteins in liver tissues (n = 2/group). E Representative TUNEL staining, IHC staining of cleaved-caspase-3 expression of liver tissues (n = 3/group). F–G Serum and hepatic levels of proinflammatory cytokines/chemokine (n = 3/group). H Representative MPO and F4/80 IF staining in liver sections (n = 3/group). All data are shown as means ± SDs. ns indicates no significance between the two indicated groups; *p < 0.05; **p < 0.01