Fig. 4

Insig2 alleviates inflammation and apoptosis during hepatic H/R. A Insig2 protein expression in primary hepatocytes extracted from mice infected with shRNA or shInsig2 AAV8. B mRNA levels of proinflammatory factors (IL-6, IL-1β, TNF-α) in hepatocytes from the indicated groups after H/R challenge (n = 3/group). C Expression of apoptosis signaling pathway proteins in hepatocytes from the indicated groups after H/R challenge (n = 3/group). D Insig2 protein expression in primary hepatocytes extracted from mice infected with Control or Insig2 overexpressing AAV8. E mRNA levels of proinflammatory factors (IL-6, IL-1β, TNF-α) in hepatocytes from the indicated groups after H/R challenge (n = 3/group). F Expression of apoptosis signaling pathway proteins in hepatocytes from the indicated groups after H/R challenge (n = 3/group). G The AML12 cells were subjected to the CCK-8 assay to determine the appropriate concentration of CoCl₂. H Establishment of Insig2 knockdown AML 12 cell lines (n=3/group). I–J The mRNA and protein levels of Bax, Bcl2 in AML12 cells from the indicated groups (n=3/group). All data are shown as means ± SDs. ns indicates no significance between the two indicated groups; *p < 0.05; **p < 0.01