Fig. 3

AAV8-mediated hepatic Insig2 overexpression ameliorates hepatic IR injury. A The AAV8 transfection efficiency was confirmed by western blot using liver tissue from mice. The WT mouse infected by AAV8 carrying Insig2 (AAV8-Insig2) or the control virus (AAV8-NC). B Levels of serum ALT and AST in AAV8-NC and AAV8-Insig2 mice under sham treatment (n = 3/group) and at 6 h after hepatic IR (n = 6/group). C Representative HE staining and quantification of necrotic areas of liver tissue from AAV8-NC and AAV8-Insig2 mice at 6 h after reperfusion or sham treatment (n = 3/group). D Western blot analysis of Bax and Bcl2 expression in livers from AAV8-NC and AAV8-Insig2 mice at 6 h after reperfusion (n = 4/group). E Representative TUNEL staining, IHC staining of cleaved-caspase-3 expression of liver tissues from AAV8-NC and AAV8-Insig2 mice at 6 h after reperfusion (n = 3/group). F The serum levels of inflammatory cytokines (IL-6, IL-1β, TNF-α) in AAV8-NC and AAV8-Insig2 mice at 6 h after reperfusion (n = 5/group). G RT-PCR analysis of the mRNA levels of IL-6, IL-1β, TNF-α, Ccl2 and Cxcl10 in AAV8-NC and AAV8-Insig2 mice at 6 h after reperfusion (n = 4/group). H Representative MPO and F4/80 IF staining (red) in liver sections of AAV8-NC and AAV8-Insig2 mice at sham or IR surgery (n = 3/group). All data are shown as means ± SDs. ns indicates no significance between the two indicated groups; *p < 0.05; **p < 0.01