Fig. 1

Insig2 expression is decreased in hepatic IR injury and negatively correlates with IR-induced liver injury in patients. A Insig2 mRNA levels in 40 donor livers of persons subjected to OLT from GSE151648 database. B Representative IHC staining of Insig2 in liver sections of persons undergone LT. C 43 human OLTs were divided into low (n = 21) and high (n = 22) Insig2 expression group of pre-OLT livers using IHC scores (IHC score < 3 as the threshold). D Pearson’s correlation coefficient between the pre-OLT livers’ IHC scores and serum ALT (sALT) at POD3 (R² = 0.3897, P = 0.0139). E sALT values in both low-Insig2 and high-Insig2 groups in OLT recipients at POD3. F Insig2 mRNA levels in livers of WT mice (1 m or 1y old) subjected to 90 min of ischemia and subsequent reperfusion for 0, 60 min from GSE10657 database (n = 3/time point). G Insig2 protein expression in livers of WT mice subjected to 1.5 h of ischemia and subsequent reperfusion for 6 h (n = 3/group) based on proteomics-sequencing. H Representative IHC staining of Insig2 in livers of mice subjected to sham treatment or hepatic IR surgery. I Western blot analysis of Insig2 protein expression in livers from WT mice subjected to sham treatment or ischemia for 1.5 h, followed by reperfusion for 0, 3 and 6 h (n = 3/group). J Western blot analysis of Insig2 protein expression in primary hepatocytes of WT mice subjected to 6 h of hypoxia and subsequent reoxygenation for 0, 3 or 6 h (n = 2/group). All data are shown as means ± SDs. ns indicates no significance between the two indicated groups; *p < 0.05; **p < 0.01