Fig. 7

Knockdown of NR3C1 activates mitophagy in ccRCC through ATF6-PINK1/BNIP3 pathway. Expression of LC3 in the control and sh-NR3C1 groups of ACHN (A) and 786-O (B) cells by immunofuorescence. (Scale bar =10 μm). The statistical results are shown in C. D Expression levels of P62, LC3, BNIP3 and PINK1 in four different groups of ACHN and 786-O cells were measured using Western blot. The four groups are: control, control+GC, sh-NR3C1 and sh-NR3C1+GC group. (GC = 1200 nM). E Expression levels of P62 and LC3 in three different groups of ACHN cells were measured using Western blot. The three groups are: control, sh-NR3C1 and sh-NR3C1+ATF6i (Ceapin-A7) group. F Expression levels of ATF6, PINK1 and BNIP3 in three different groups of ACHN and 786-O cells were measured using Western blot. Three groups were: control, sh-NR3C1, sh-NR3C1+ATF6i. Data presented as mean  ±  SD with three replicates. *P < 0.05; ***P <  0.001. LC3, microtubule associated protein light chain 3; GC, glucocorticoids; ATF6i, Ceapin-A7 as ATF6 inhibitor