Fig. 4

Lipidomic profiles in ccRCC cells after NR3C1 knockdown. A Metabolites donut charts of the control and sh-NR3C1 groups. Different colors represent different metabolite classification. B Bubble chart showed the differences in lipid metabolites levels between the control and sh-NR3C1 groups. Each point in the figure represents a different lipid, with the color of the point corresponding to different lipid subclasses and the size of the point indicating p after − log10 conversion. C Bar graphs illustrate the differences in expression of major lipid components between the two groups. D Chain unsaturation distribution of PC in the control and sh-NR3C1 groups. The x-axis represents the unsaturation of the carbon chain, while the y-axis represents peak intensity. The numbers on the x-axis indicate the number of unsaturated fatty acids present in the carbon chain. E Bar graphs illustrate the differences in expression of classical polyunsaturated fatty acids profiles between the control and sh-NR3C1 groups. Data presented as mean  ±  SD with three replicates. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. PC, phosphatidylcholine; SM, sphingomyelin; PE, phosphatidylethanolamine; Cer, ceramides; PS, phosphatidylserine; PI, phosphatidylinositol; TG, triglyceride; CerG1, monogylcosylceramide; DG, diglyceride; LPC, lyso-phosphatidylcho line; AA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid