Fig. 3

Knockdown of NR3C1 inhibits the migration of ccRCC. The effect of NR3C1 knockdown on the migration of ACHN (A) and 786-O cells (B) were assessed using wound healing assays. The concentrations of GC were: 400nM, 800 nM and 1200 nM. Images were acquired at 0, 8 and 16h. (Scale bar =700 μm). Bar graphs represent the changes in scratch healing area in ACHN (C) and 786-O cells (D) at 8 and 16h. E The effect of NR3C1 knockdown on the migration of ccRCC cells were assessed using Transwell assays. Images were acquired at 12h, and are representative of three independent experiments. (GC=1200 nM; scale bar =100 μm). F Bar graphs represent the results of Transwell assays in ACHN and 786-O cells. Data presented as mean  ±  SD with three replicates. *P <  0.05; **P <  0.01; ***P <  0.001; ****P <  0.0001. GC, glucocorticoids