Fig.1

The expression of NR3C1 is elevated in ccRCC. A Expression of NR3C1 mRNA in ccRCC (n = 533) and adjacent normal (n = 72) tissues in the TCGA-KIRC cohort. B Expression of NR3C1 protein in ccRCC (n = 110) and adjacent normal (n = 84) tissues in the CPTAC database. C Meta-analysis of NR3C1 expression differences across 7 analyses about ccRCC in the Oncomine database. Expression of NR3C1 was examined in ccRCC and peritumoral tissues using RT-qPCR (n = 8) D and Western blot (n = 10) E. F Expression of NR3C1 was examined in ccRCC cells and normal renal tubular epithelial cells (HK2) using RT-qPCR. Four types of ccRCC cells are included: 786-O, ACHN, A498 and UMRC6. G Expression of NR3C1 was analyzed in 786-O, ACHN and HK2 cells using Western blot. Data presented as mean ± SD with three replicates. Ns, no significant difference; ***P < 0.001; ****P < 0.0001. ccRCC, clear cell Renal Cell Carcinoma; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; CPTAC, Clinical Proteomic Tumor Analysis Consortium; Oncomine, Oncomine tumor chip database; T, ccRCC tissues; P, peritumoral tissues