Fig. 3

Effect of CpG-ODNs regulating PARP1 expression on in vitro RKI cell model. A RT-qPCR to detect the relative expression of PARP1 mRNA in the kidney tissue of RKI mice treated with CpG-ODN2006 or CpG-ODN2216. B RT-qPCR to detect the relative expression of PARP1 mRNA in radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination. C CCK-8 assay to detect the viability of radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination. D The Caspase activity in radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination as determined with enzymatic method. E Immunofluorescence assay to detect the γH2AX-positive cell ratio of radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination. Blue indicated DAPI fluorescence labeled nucleus, and green indicated labeled γH2AX. F Levels of oxidative stress-related factors (SOD, CAT, GSH, and GPx) in radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination. G MDA levels in radiation-treated HK-2 cells after CpG-ODN or sh-PARP1 treatment alone or in combination. * P < 0.05 versus control mice or control cell. # P < 0.05 versus RKI mice, radiation-treated HK-2 cells or radiation-treated HK-2 cells treated with sh-NC. & P < 0.05 versus radiation-treated HK-2 cells treated with CpG-ODN2006. All cell experiments were repeated 3 times. n = 8 mice for each treatment