Fig. 9

Validation of JUN and ZFP36 expression at the protein level from clinical synovial samples. A IHC of JUN. B IHC of ZFP36. C IHC of GPX4. D Relative protein levels of JUN, ZFP36 and GPX4 from IHC. E Relative mRNA expression levels of JUN and ZFP36. F Western blotting assay of JUN, ZFP36, GPX4, and GAPDH. G Statistical analysis of the results of western blotting assay. IHC, immunohistochemistry. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 versus K–L Grade 2; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus K–L Grade 3