Fig. 6

AC142119.1 recruits WDR5 protein to activate the transcription of MYCN. A ChIRP assay was carried out to evaluate the enrichment of AC142119.1 transcript on the promoter of MYCN. B ChIP assay was executed to detect the H3K4me3 modification of MYCN promoter. C, D The expression of mRNA (C) and protein (D) level of MYCN was assessed by qRT-PCR and western blot, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance