Fig. 5

AC142119.1 directly binds with WDR5 in NB cells. A Silver staining of the SDS-PAGE gel showing the separated proteins pulled down by biotin-labeled AC142119.1 RNA. B Western blot analysis of WDR5 in protein samples captured by RNA pull-down assay. C RIP assays to validate the interaction between endogenous AC142119.1 transcript and WDR5 in SK-N-DZ and IMR-32 cells. D, E Interaction profile (D) and matrix (E) between AC142119.1 transcript and WDR5 protein predicted by catRAPID database. F Schematic diagram of full-length and truncated AC142119.1 RNA. G RNA pull-down assays were conducted with biotin labeled full-length and truncated AC142119.1 transcript. The precipitated proteins were analyzed by western blot. H RNA EMSAs to determine the domains of WDR5 responsible for the interaction with AC142119.1 RNA. **P < 0.01