Fig. 2

AC142119.1 is elevated in MYCN-amplified NB tissues and positively correlated with the poor survival in NB patients. A qRT-PCR to analyze the expression of AC142119.1 in MYCN-amplified and non-amplified NB cell lines. B, C The mRNA (B) and protein (C) levels of MYCN in MYCN-amplified and non-amplified NB cell lines were determined by qRT-PCR and western blot, respectively. D, F Relative expression of AC142119.1 (D) and MYCN (F) in NB patients with or without stage IV. E, G Relative expression of AC142119.1 (E) and MYCN (G) in NB patients with or without high-risk. H Pearson correlation analysis between the expression of AC142119.1 and MYCN in 55 NB tissues. I The survival probability of NB patients was analyzed by Kaplan–Meier method. The patients were categorized into low and high group according the median of AC142119.1 expression in all NB patients. *P < 0.05, **P < 0.01, ***P < 0.001