Fig. 7

Effects of CTLA4⁺ T cells-secreted S100A4 on the stemness phenotype of TNBC cells. A Heatmap of DEGs in CTLA4⁺ T cells. Red represents upregulated genes, and green represents downregulated genes. B and C S100A4 expression change in T cells and the culture supernatants of T cells after co-culture with MDA-MB-231 or BCSCs-231 cells as detected by RT-qPCR and ELISA. D Flow cytometry to determine the effect of co-culture of MDA-MB-231-T or BCSCs-231-T with T cells on the proportion of CD44⁺ and CD24⁻ MDA-MB-453 cells. E Flow cytometry to determine the effect of exogenous hrS100A4 on the proportion of CD44⁺ and CD24⁻ MDA-MB-231 and MDA-MB-453 cells. ^ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. All cell experiments were repeated three times