Fig. 6

In vivo verification of the involvement of TN-BCSCs in immune escape of TNBC through CHI3L1/MAF. A Growth of MDA-MB-231 and BCSCs-231 orthotopic B.C. transplanted tumors. The tumor growth curve drawn based on the relative bioluminescence intensity is on the left. The bioluminescence intensity and tumor size at the end of the experiment are shown on the right side. B and C Detection of CHI3L1, MAF and CTLA4 expression in tumor tissues by RT-qPCR and Western blot. D and E, Detection of CTLA4⁺ T cell proportion and CD8⁺ T cytotoxicity (GzmB and Ki67) in tumor tissues by flow cytometry (n = 8). F Growth of MDA-MB-453 orthotopic B.C. transplanted tumor. The tumor growth curve drawn based on the relative bioluminescence intensity is on the left. The bioluminescence intensity and tumor size at the end of the experiment are shown on the right side (n = 8). G Flow cytometry to detect the proportion of BCSCs in MDA-MB-453 orthotopic B.C. transplanted tumors in mice treated with hrCHI3L1 or sh-MAF-1 (n = 8). H and I, Detection of CTLA4⁺ T proportion and CD8⁺ T cytotoxicity in MDA-MB-453 orthotopic B.C. transplanted tumor in mice treated with hrCHI3L1 or sh-MAF-1 by flow cytometry. ^ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001