Fig. 4

Effects of TN-BCSCs on CTLA4 expression in T cells. A RT-qPCR and Western blot to measure CTLA4 expression in T cells after co-culture with MDA-MB-231 or BCSCs-231 cells. B Representative results of CTLA4⁺ (on the cell surface) T cell proportion changes after co-culture (left) and quantitative chart (right). C Scatter plot showing the expression distribution of T cell-related transcription factors of CTLA4 predicted through the Cistrome D.B. database in cell subsets. D distribution of the predicted transcription factors of CTLA4 (ETS1, MAF and PSIP1) in the scRNA-seq data. E Correlation between MAF and CTLA4 expression in the T cell subset of the scRNA-seq data. F RT-qPCR and Western blot to detect CTLA4 expression change after co-culture of T cells with MAF knockdown and BCSCs-231 cells. G Representative results (left) and quantitative statistical chart (right) of the change in the proportion of CTLA4⁺ T cells after co-culture of T cells with MAF knockdown and BCSCs-231 as determined by flow cytometry. ^ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Cell experiments were repeated three times