Fig. 6

POSTN positively regulates cells adhesion, migration and invasion. ShPostn, knocking down Postn; OePostn, overexpressesing Postn; rPN, recombinant POSTN protein. A and B plots were from RCF and H9c2 cell adhesion experiments, respectively. Among them, ☆ Compared with 0 μg/L rPN group, the number of adhered cells in the 100 μg/L rPN group was increased significantly, P < 0.05. ^✲ Compared to the 0 μg/L rPN group, the number of adherent cells in the OePostn group was significantly higher than that in the 200 μg/L rPN group. Fig C is the results of RCF cell migration assay. ^α After 6 h, the migration ability of OePostn group was significantly higher than that of the control, NC, ShPostn and rPN group, P < 0.05; ^βafter 12 h, the migration ability of overexpression group was significantly higher than that of control, NC, ShPostn and rPN group, P < 0.05; ^γ after 12 h, the migration ability of ShPostn + 100 μg/L rPN was significantly higher than that of ShPostn and NC group, ^P < 0.05; ^δafter 12 h, the migration ability of ShPostn + 200 μg/L rPN was significantly higher than that of control, ShPostn and ShPostn + 100 μg/L rPN group, P < 0.05; ^κafter 24 h, the migration ability of ShPostn group was significantly lower than that of other groups, P < 0.05. As shown in Fig. D, which is Transwell's experiment,☆ indicated that there was significant difference between 0 μg/L rPN and 200 μg/L rPN in ShPostn group. *P < 0.05, **P < 0.001