Fig. 7

GSDMD is overexpressed in SKCM tissues as a protective gene by inhibiting the proliferation, migration and invasion abilities of SKCM cells. A Multivariate Cox regression analysis was performed on the genes derived from the univariate Cox regression analysis. B Comparison of mRNA levels of GSDMD in SKCM and normal tissues from TCGA and GTEx databases. C Representative images of GSDMD immunohistochemistry and its quantification. D WB and WB quantification of GSDMD protein levels in A375 and A2058 cell lines infected with scramble or GSDMD overexpression lentivirus, or treated with SB525334. E Cell apoptosis in each group of A375 and 2058 cell lines was measured by FCM assay. F Cell viability in each group of A375 and 2058 cell lines was determined by CCK8 assay. G–H Ki67 immunofluorescence staining in A375 (G) and A2058 (H) cell lines and its quantification (Scale bar = 100 µm). I–J The migration ability of A375 (I) and A2058 (J) cell lines was detected by Transwell assay (Scale bar = 100 µm). K–L The invasion ability of A375 (K) and A2058 (L) cell lines was detected using Matrigel-coated Transwell cell culture chambers (Scale bar = 100 µm). All results are presented as the mean ± SD, ns: p > 0.05; ***p < 0.001