Fig. 6
GD-induced RIPK3 activation affects Aβ levels and increases neuronal loss in the APP/PS1 mice. A The levels of soluble A and B and insoluble C and D Aβ40 and Aβ42 in mouse hippocampal samples measured by ELISA. E β-Secretase activity in the hippocampus of mice. F Western blotting analysis of BACE1 and Aβ expression in the hippocampus in the indicated mice. One-way ANOVA followed by Tukey test. *p < 0.05. The values are presented as the mean ± SEM (n = 6/group). G Primary microglia from APP/PS1 mice were treated with oligomeric Aβ1–42 and then treated with T3 or GSK872. Western blotting analysis was used to examine RIPK3, MLKL, p-RIPK3, and p-MLKL protein levels. H The levels of Aβ42 in the supernatant were evaluated by ELISA. One-way ANOVA followed by Dunnett’s test. *p < 0.05 compared to control cell. The values are presented as the mean ± SEM. N.S.: no significance. I APP/PS1 mice were treated with Ad-TSHR289 or Ad-Control in the presence of either vehicle or GSK872. Double immunostaining of p-MLKL and NeuN in the cortex