Fig. 5

GD induces the necroptosis activation in the hippocampus of APP/PS1 mice. A Western blotting analysis of RIPK3/MLKL and Aβ in the hippocampus of mice. B (Top) Representative images of coimmunostaining of p-RIPK3 (red), p-MLKL (green), and Aβ (yellow) in the hippocampus of GD model mice. Scale bar, 100 μm. (Bottom) Higher magnification images. Scale bar, 20 μm. C Schematic drawing of the experimental schedule: APP/PS1 mice were treated with Ad-TSHR289 or Ad-Control in the presence of either vehicle or GSK872. D Western blotting analysis of p-RIPK3 and p-MLKL levels in the hippocampus of the indicated mice. E Immunostaining of p-RIPK3 at higher magnification in the hippocampus of the indicated mice. Scale bar, 200 μm. F Immunostaining of p-MLKL at higher magnification in the hippocampus of mice. Scale bar, 200 μm. G TEM of the hippocampus of the indicated mice. Scale bar, 5 μm or 2 μm. H Immunoprecipitation was performed to assess the interaction of p-RIPK3 with MLKL