Fig. 2

GD increases Aβ accumulation and neuronal loss in APP/PS1 mice. A Congo red staining of Aβ plaque (arrow) and quantification in the hippocampus. Scale bar = 200 μm. B Immunostaining of Aβ protein at higher magnification at hippocampus of the mice (n = 4). Scale bar, 100 μm and 50 μm. C Representative images of NeuN immunostaining at higher magnification in the cortex of the mice (scale bar, 50 μm and 20 μm). D Representative images of Nissl staining in the hippocampus (scale bar, 100 μm and 20 μm) and cortex (scale bar, 100 μm) of the indicated mice. Unpaired Student’s t-test, *p < 0.05 compared to APP/PS1 mice. The values are represented as mean ± SEM (n = 6/group). N.S. no significance