Fig. 7

AFAP1L1 knockdown promotes ocular neovascularization in vivo. A–D IsoB4 staining of whole-mount retinas from OIR mice at P17 intravitreously injected with NC-ECKD, AFAP1L1-ECKD #1 or AFAP1L1-ECKD #2 (Scale bar: 1 mm) (n = 4 mice for each group); the purple area indicates avascular area, and the white area indicates NVTs (A). Quantification of neovascular tuft area and avascular area in OIR mice (B). The red areas highlighted vascular area especially filopodia in the anterior retina of the nonperfusion area (C). Quantification of the number and length of filopodia (green asterisk) respectively. (Scale bar: 40 μm) (n = 4 mice for each group) (D). E–F IsoB4 staining of whole-mount retinas from L-CNV mice intravitreously injected with NC-ECKD, AFAP1L1-ECKD #1 or AFAP1L1-ECKD #2. (n = 4 mice for each group) (E). Quantification of size of CNV lesions in L-CNV mice (Scale bar: 200 μm) (F). G–H All the cornea samples of S-CNV mice subconjunctivally injected with NC-ECKD, AFAP1L1-ECKD #1 or AFAP1L1-ECKD #2 were collected for vascular visualization by slit lamp. (n = 4 mice for each group) (G). Quantification of size of CNV lesions in S-CNV mice H (Scale bar: 400 μm). P values were calculated by one-way ANOVA with Bonferroni's post hoc test B, D, F, H. Error bars represent the mean ± SD. *: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0.001; n.s.: no significance