Fig. 6

AFAP1L1 knockdown promotes tumor growth and angiogenesis in vivo. A–C Xenografts were established by injecting A549 cells subcutaneously on the dorsal flank of BALB/c nude mice, and intratumoral administration of NC-ECKD, AFAP1L1-ECKD #1 or AFAP1L1-ECKD #2 was performed once the tumor length reached 100 mm.³ (n = 4 mice for each group). Representative images were shown (A); quantification of tumor growth curves of xenograft in nude mice (Day 0 represents the time of the initial AAV injection) (B); quantification of tumor weights of xenograft in nude mice (C). D–E Xenografts at the end points were sliced into sections for vascular visualization (n = 4 mice for each group); the sections were labeled with IsoB4 (red) and the nuclei stained with DAPI (blue) (D); quantification of IsoB4 stained vessels in the sections of xenografts (E). P values were calculated by one-way ANOVA with Bonferroni’s post hoc test (B, C, E). Error bars represent the mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001