Fig. 4

AFAP1L1 is associated with vascular endothelial features in cancer and retina. A The heatmap represents the correlation of AFAP1L1 expression with immune infiltration level in diverse cancer types. B The heatmap represents the correlation between AFAP1L1 expression level and immune infiltration of endothelial cells and several known vascular endothelial cell markers in diverse cancer types using the TIMER2 database based on EPIC, MCPCOUNTER and XCELL algorithms. C–D Clustering is performed by the cell type (C), the patient types or the tumor types D and visualized using Tsne algorithm from the publicly available scRNA-seq data of lung tumor endothelial cell through the Seruat package. E Cross validation of the identified EC marker genes (PECAM1), tip cell marker genes (DLL4 and CXCR4) and AFAP1L1 from the publicly available scRNA-seq data of lung tumor endothelial cell through the Seruat package. F The grouped bar charts show the expression of AFAP1L1 in total and different types of lung cancer. G The stacked bar charts show the expression of AFAP1L1 in different lung cancer types and different cell types. H Colocalization of AFAP1L1 and tip cell marker (DLL4) (DLL4: blue; AFAP1L1: green; IsoB4: red) in the anterior end of the retinal vascular. Scale bar: 25 μm. (n = 4 independent experiments)