Fig. 2

Experimental paradigm for measuring PER efficacy in a preclinical nonadherence paradigm. A Adult male Sprague Dawley rats (150–200 g) underwent repeated low dose kainic acid (KA)-induced status epilepticus (SE). Approximately 3–4 weeks following cessation of SE, rats were implanted with cortical EEG electrodes and allowed to fully recover before starting on PCBO chow at 60 g/kg/day (15 g/kg, q.i.d. p.o.). Baseline seizure frequency, burden and severity were recorded for 4 weeks; upon reaching an average > 1 Racine stage 3 or higher seizure/week animals randomly assigned to receive PER 100% of the time (average daily dose: 10 mg/kg/day) or randomly 50% of the time (average daily dose: 5 mg/kg/day) for a 4-week treatment phase. B Schematic depicting meal delivery over the course of a week (q.i.d. × 7 days) for 50% nonadherent treatment group. Red dots indicate medicated meal, black dot represents placebo meal