Fig. 1

Ven activates GSDME-mediated pyroptosis in AML cells. A The chemical formula of Ven. B Viability of five AML cell lines treated with different concentrations of Ven for 24 h. C Micrographs of the five AML cell lines treated with Ven. The arrowheads indicate blebbed pyroptotic cells; scale bar: 50 μm. D Release of LDH into the culture supernatant was detected by an LDH assay kit. E Ven-treated Molm-3 and THP-1 cells were subjected to Hoechst/PI staining. Nuclei were stained blue with Hoechst, and red with PI. F AML cells were treated with Ven, and cell lysates were then subjected to western blot analysis to confirm GSDM protein expression and cleavage. G Protein levels of full-length (FL) and N-terminal of GSDME, pro-caspase-3 (Pro-CASP3) and cleaved caspase-3 (CL-CASP3), pro-PARP1 and cleaved PARP1 in Ven-treated Molm-13 cells, THP-1 cells, and primary cells from AML patients. β-actin was used as an internal control for western blotting