Fig. 8
From: Tamoxifen exerts anti-peritoneal fibrosis effects by inhibiting H19-activated VEGFA transcription
Roles of ESR1 and H19 RNA in predicting peritoneal function. a HPMCs were cultured from PD effluent of two patients (1-month duration and 5-year duration). E-cadherin and ESR1 expressions were detected by immunofluorescence. Scale Bar = 20 μm. b Images of Western Blot analysis showing ESR1 protein levels of PD effluent cell lysates from patients (PD duration: 6 months, 2 years, and 5 years). c Localization of H19 in HPMCs was presented by RNA FISH. Scale Bar = 50 μm. d and e A positive linear correlation between ESR1 (or H19) RNA levels in human exfoliated mesothelial cells and D/P Cr. Pearson correlation was conducted. HPMCs human peritoneal mesothelial cells, D/P Cr dialysate/plasma ratio of creatinine