Fig. 7

The pro-fibrotic effect of H19 can be partially blocked by interfering with VEGFA a Validation of the siVEGFA efficiency in MeT-5A cells by qRT-PCR. n = 3. *P < 0.05, **P < 0.01 vs. siNC group. b Images showing the morphology of LV-H19- or LV-NC-infected MeT-5A cells transfected with siVEGFA-2 or siNC. c CCK8 showing cell viability. n = 3. **P < 0.01 vs. LV-NC + siNC group, ^##P < 0.01 vs. LV-H19 + siNC group. d and e Western blot analysis of EMT markers and pathways activated in the EMT process and fibrosis. f ELISA showing VEGFA in the cell supernatant. n = 3. **P < 0.01 vs. LV-NC + siNC group, ^##P < 0.01 vs. LV-H19 + siNC group. g and h EdU assay for the proliferation of LV-H19- or LV-NC-infected MeT-5A cells and quantification of EdU-positive cells. Scale bars = 100 μm. n = 3. **P < 0.01 vs. LV-NC + siNC group, ^##P < 0.01 vs. LV-H19 + siNC group. i and j TUNEL Bright Red test showing apoptosis and quantification of TUNEL-positive cells of LV-H19- or LV-NC-infected MeT-5A cells. n = 3. **P < 0.01 vs. LV-NC + siNC group, ^##P < 0.01 vs. LV-H19 + siNC group. Scale bars = 100 μm. n = 3. One-way ANOVA was performed followed by Dunnett's T3