Fig. 5

Interference with H19 inhibited PD-related peritoneal fibrosis in the mouse. a qRT-PCR to validate the efficiency of siRNAs targeting mouse H19. The nanomaterial-encapsulated modified siRNAs were injected into the peritoneal cavity of mice for 24 h. Then, the peritoneal cells were extracted by EDTA-trypsin digestion. Total RNA was extracted by the phenol/chloroform method, followed by qRT-PCR. We chose siH19-3 for the following experiment. n = 6 mice. Values were performed normality test followed by Dunn’s comparisons test. b Masson-stained images showed peritoneal collagen deposition in mice. Scale bars = 100 μm. c Relative quantification of mouse peritoneal thickness by ImageJ. n = 6. **P < 0.01 vs. siNC group. ^##P < 0.01 vs. PD + siNC group. d and e Peritoneal function test of ultrafiltration failure (UF) measurements and glucose transfer (MTG) in the mouse. n = 6. **P < 0.01 vs. siNC group. ^##P < 0.01 vs. PD + siNC group. f Western blot analysis of EMT and fibrosis markers in mouse peritoneal tissues. Values are the mean ± SD. The pairwise comparison between groups was tested by Dunnett's T3 following One-way ANOVA (c-e)