Fig. 4

The pro-fibrotic effect of HG was reversed by siH19, while aberrantly elevated H19 exacerbated fibrotic pathological changes in MeT-5A cells. a qRT-PCR to validate the efficiency of siH19 in MeT-5A cells. n = 3. **P < 0.01 vs. siNC group. b Images of MeT-5A cell morphology after transfection with siNC or siH19. c CCK8 assay showed the viability of MeT-5A cells transfected with small interfering RNAs with or without HG stimulation. n = 3. **P < 0.01 vs. siNC group, ^#P < 0.05 vs. siNC + HG group. d Western blot analysis of EMT and fibrosis markers in MeT-5A cells transfected with siNC or siH19 and stimulated with HG. e ELISA to detect the VEGFA protein in the cell supernatant of MeT-5A cells. n = 3. **P < 0.01 vs. siNC group, ^##P < 0.01 vs. siNC + HG group. f qRT-PCR to validate the efficiency of LV-H19 compared to LV-NC. n = 3. **P < 0.01 vs. LV-NC group. g Morphology images of MeT-5A cells infected with LV-H19 or LV-NC. h CCK8 assay showing cell viability. n = 3. **P < 0.01 vs. LV-NC group. i Western blot analysis of EMT and fibrosis markers. j ELISA to detect the VEGFA protein in LV-H19- or LV-NC-infected MeT-5A cell line supernatant. n = 3. **P < 0.01 vs. LV-NC group. Values are the mean ± SD. The pairwise comparison between groups was tested by Dunnett's T3 following One-way ANOVA