Fig. 3

High glucose promoted H19 expression through ESR1, and TAM repressed this process. a The expression of H19 in MeT-5A cell lines of the CON, HG, and HG + TAM groups by qRT-PCR. n = 3. **P < 0.01 vs. CON group, ^#P < 0.05 vs. HG group. b qRT‒PCR to detect the expression of H19 in the MeT-5A cell line under stimulation with different glucose concentrations. n = 3. **P < 0.01 vs. 0% glucose group. c mH19 expression in mouse peritoneal primary cells at different glucose concentrations by qRT-PCR. n = 3. **P < 0.01 vs. 0% glucose group. d mH19 levels in mouse peritoneal primary cells of the CON, HG, and HG + TAM groups. n = 3. **P < 0.01 vs. CON group, ^##P < 0.01 vs. HG group. e qRT-PCR to detect H19 expression in the peritoneums of clinical patients. n = 6 samples. **P < 0.01 vs. CON group. f Western blot analysis of ESR1 nuclear protein expression in the CON, HG, and HG + TAM groups in the MeT-5A cell line. g Relative quantification of ESR1 protein level according to Western Blot images. n = 3. **P < 0.01 vs. CON group, ^##P < 0.01 vs. HG group. h Western blot analysis of the nuclear expression of Esr1 in mouse peritoneal primary cells in the CON, HG, and HG + TAM groups. i Quantification of ESR1 protein level according to Western Blot images in murine peritoneal cells. n = 3. *P < 0.05 vs. CON group, ^#P < 0.05 vs. HG group. j and k ESR1 enrichment levels in the two binding sites of H19 promoter in MeT-5A cells by ChIP assay. The ChIP assay was used to obtain DNA fragments bound to the ESR1 antibody, followed by the design of primers based on the predicted binding sites for qPCR quantification. Enrichment was normalized to input controls. Primers for ChIP were available in Additional file 1: Table 3. n = 3. **P < 0.01 vs. CON/ESR1 group, ^##P < 0.01 vs. HG/ESR1 group. There is no data significance among the IgG group. l ESR1 binding sites of the H19 promoter mice. n = 3. **P < 0.01 vs. CON/Esr1 group, ^##P < 0.01 vs. HG/Esr1 group. There is no data significance among the IgG group. m Luciferase activity in MeT-5A cells transfected with luciferase reporters containing the wild-type or mutant H19 promoter region. The two binding points were both mutated in the reporter plasmid. The results are shown as the ratio of firefly luciferase activity to Renilla luciferase activity. n = 3. **P < 0.01 vs. CON/WT group, ^##P < 0.01 vs. HG/WT group. There is no data significance among the MUT group. Values are the mean ± SD. Two-way ANOVA followed by Tukey’s post-test. H19 long noncoding RNA H19, mH19 H19 in mice