Fig. 2

Tamoxifen reversed PD-related peritoneal fibrosis. a Masson-stained images showed peritoneal collagen deposition in the mouse model. The CON group received a daily intraperitoneal injection of saline (0.1 ml/g). The PD group received an equal volume of 4.25% daily. Mice in the PD + TAM group received an injection of PDF and tamoxifen citrate treatment, 10 mg/kg, daily, by gavage. n = 6 mice. Scale bars = 100 μm. b Relative quantification of peritoneal thickness in the mouse model. n = 6. **P < 0.01 vs. control group, ^##P < 0.01 vs. PD group mice. c and d Peritoneal function test of UF measurements and mass transfer of glucose (MTG). n = 6. **P < 0.01 vs. CON group, ^##P < 0.01 vs. PD group. e Morphology of mouse peritoneal primary cells. Scale bars = 100 μm. f CCK-8 assay showed the viability of mouse peritoneal primary cells under HG stimulation with or without tamoxifen treatment. n = 3. *P < 0.05 vs. 48 h HG group, ^##P < 0.01 vs. 72 h HG group. g Western blot analysis of EMT and fibrosis markers in mouse peritoneal primary cells. h Statistical data analysis of protein Western Blot results. n = 3. *P < 0.05, **P < 0.01 vs. CON group, ^#P < 0.05, ^##P < 0.01 vs. HG group. i VEGFA ELISA of mouse peritoneal primary cells. n = 3. *p < 0.05, **P < 0.01 vs. CON group, ^#P < 0.05, ^##P < 0.01 vs. HG group. j Images of MeT-5A cell morphology captured by microscopy. Scale bars = 100 μm. k CCK8 assay showed the viability of MeT-5A cells with or without tamoxifen treatment. n = 3. *P < 0.05, vs. 48 h HG group, ^##P < 0.01 vs. 72 h HG group. l Western blot analysis of EMT and fibrosis markers in MeT-5A cells. m Statistical data analysis of protein Western Blot results. n = 3. *P < 0.05, **P < 0.01 vs. CON group, ^#P < 0.05, ^##P < 0.01 vs. HG group. n VEGFA ELISA of MeT-5A cell supernatant. n = 3. **P < 0.01 vs. CON group, ^#P < 0.05 vs. HG group. TAM tamoxifen, HG 4.25% glucose, UF ultrafiltration failure, MTG mass transfer of glucose. Cellular studies are performed three times. Values are the mean ± SD. The pairwise comparison between groups was tested by Dunnett's T3 following One-way ANOVA