Fig. 7

MCL assuages BRG1-induced fibrotic responses by binding to the residue of asparagine 1540 of BRG1. a Pull-down experiment with a biotin-MCL indicated that MCL directly bound to BRG1 in RPMCs lysates. b Pull-down experiment with a biotin-MCL indicated that MCL directly bound to BRG1 in HMrSV5 cell lysatases. c Cellular thermal shift assay confirmed the binding of MCL to BRG1 in HMrSV5 cells. d Pull-down experiment with a biotin-MCL indicated that MCL directly bound to BRG1 C-terminal region. e Molecular docking of MCL with the protein BRG1. f Pull-down experiment with a biotin-MCL indicated that BRG1 N1540A mutant abrogated the interaction between MCL and BRG1. g Co-immunoprecipitation demonstrated that the interaction between BRG1 and H3K14ac was decreased after the mutation of BRG1 N1540. After transfection with BRG1 full length plasmid or N1540A mutant plasmid, cell lysates were IP with anti-BRG1, followed by IB with anti-BRG1 and anti-H3K14ac. h, i Cells were transfected with BRG1 full length plasmid, N1540A mutant plasmid or vector plasmid, and then were treated with DMSO or MCL. Western blot analyses of expression of a-SMA, Collagen I, Fibronectin, TGF-β1 and P-Smad3 in different groups are presented