Fig. 5

Administration of DMAMCL ameliorates BRG1 overexpression-induced peritoneal fibrosis in a mouse model of PD. a Experimental design. Red arrows indicate the injection of control (Vector) or BRG1 overexpression plasmid (pBRG1). Orange line indicates the administration of DMAMCL or saline. b, c Representative micrographs show collagen deposition by Masson’s trichrome staining (b) and quantitative analysis (c) in the parietal peritoneum in different groups as indicated. Scale bar, 50 μm. *P < 0.05 versus group I (vector + saline), ^#P < 0.05 versus group II (vector + PDF + saline). ^&P < 0.05 versus group III (pBRG1 + PDF + saline). n = 5–6 per group. d–h Western blot analysis showed visceral peritoneum expression of BRG1, Fibronectin, Collagen I, and a-SMA in different groups as indicated. Representative Western blot (d) and quantitative data on the relative abundance of BRG1 (e), a-SMA (f), Collagen I (g), and Fibronectin (h) proteins are presented. *P < 0.05 versus group I (vector + saline), ^#P < 0.05 versus group II (vector + PDF + saline). ^&P < 0.05 versus group III (pBRG1 + PDF + saline). n = 5–6 per group. i Representative micrographs show Fibronectin (top), and Collagen I (bottom) staining in different groups as indicated. Fibronectin and Collagen I were detected by immunohistochemical staining. Scale bar, 50 μm. j–k Western blot analysis showed visceral peritoneum expression of TGF-β1,P-Smad2 and P-Smad3 in different groups as indicated. Representative Western blot (j) and quantitative data (k) on the relative abundance of TGF-β1, P-Smad2 and P-Smad3 are presented. *P < 0.05 versus group I(vector + saline), ^#P < 0.05 versus group II(vector + PDF + saline). ^&P < 0.05 versus group III (pBRG1 + PDF + saline). n = 4–6 per group