Fig. 4

MCL inhibits BRG1-induced fibrotic responses and TGF-β1-Smad2/3 pathway in vitro. a The structure of MCL. b, d, e, f, g Western blot analyses show that MCL reduced BRG1-induced expression of Vimentin, BRG1, Collagen I and Fibronectin in RPMCs. Representative Western blot (b) and quantitative data on the relative abundance of proteins (d–g) in different groups are presented. *P < 0.05 versus Vector. ^#P < 0.05 versus pBRG1. c Representative micrographs show that MCL reduced Smad3 and Smad2 nuclear accumulation induced by TGF-β1. Scale bar, 10 μm. h–j Western blot analyses show that MCL reduced TGF-β1-induced phosphorylation of Smad2 and Smad3. Representative Western blot (h) and quantitative data (i, j) in different groups are presented. *P < 0.05 versus conrol group, ^#P < 0.05 versus TGF-β1 group