Fig. 3

BRG1 activates TGF-β1-Smad2/3 signaling pathway in vitro. a, c Western blot analyses show that BRG1 overexpression induced phosphorylation of Smad2 and Smad3.Western blot (a) and quantitative data (c) in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group. *P < 0.05 versus Vector, ^#P < 0.05 versus Vector in the presence of TGF-β1. (b, d) Western blot analyses show that knockdown of BRG1 by siRNA strategy reduced TGF-β1-induced phosphorylation of Smad2 and Smad3. Western blot (b) and quantitative data (d) in different groups are presented. *P < 0.05 versus siNC, ^#P < 0.05 versus siNC in the presence of TGF-β1. e Representative micrographs show that knockdown of BRG1 repressed Smad2 and Smad3 nuclear accumulation induced by TGF-β1. Scale bar, 10 μm. f, g Western blot shows the distribution of Smad2 and Smad3 in cytosolic and nuclear fractions of different groups as indicated. GAPDH and Histone H3 were used to normalize cytosolic and nuclear fractions, respectively. Western blot (f) and quantitative data (g) in different groups are presented. *P < 0.05 versus siNC, ^#P < 0.05 versus siNC in the presence of TGF-β1