Fig. 2

BRG1 promotes fibrotic responses in vitro. a–e HMrSV5 cells were transfected with control vector (Vector)or BRG1 expression plasmid (pBRG1) for 48 h. Western blot analyses show that overexpression of BRG1 induced the expression of Fibronectin,Collagen I and a-SMA expression. Representative Western blot (a) and quantitative data (b–e) in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group. *P < 0.05 versus Vector. f RPMCs were transfected with control vector (Vector) or BRG1 expression plasmid (pBRG1) for 48 h. Western blot analyses show that overexpression of BRG1 induced the expression of TGF-β1 but reduced the E-cadherin expression. Numbers (1, 2 and 3) represent different samples in a given group. Triangle represents incremental plasmid dosage (2 μg, 3 μg and 4 μg/plate). g Representative micrographs show that BRG1 overexpression promoted Fibronectin(top) and a-SMA(bottom) expression. Scale bar, 50 μm. h, i Western blot analyses show that BRG1 interference by siRNA strategy reduced TGF-β1-induced expression of Collagen I, Vimentin, Fibronectin and restored E-cadherin expression. Representative Western blot (h) and quantitative data (i) in different groups are presented. *P < 0.05 versus siNC, ^#P < 0.05 versus siNC in the presence of TGF-β1. j Representative micrographs show that knockdown of BRG1 repressed a-SMA (top) and Fibronectin(bottom) expression induced by TGF-β1. Scale bar, 50 μm. k Western blot analyses show the effect of BRG1 inhibitor PFI3 on TGF-β1-induced expression of Collagen I, a-SMA, Vimentin. Numbers (1, 2 and 3) represent different samples in a given group